To start loading a gene, select "Candidate > Load..." as pictured below:
Step 1 - Search
In the "Open a new Candidate" screen that pops up, enter the search string for your gene, select a species from the drop-down list, and click "Go". The search will continue until it has found all related genes. You may start a new search simply by inserting a new search string and hitting "Go". The search string may consist of any of the following:
| ID Type | Example |
| Official Gene Symbol | ss18 |
| Ensembl Gene ID | ENSG00000141380 |
| Ensembl Transcript ID | ENST00000269137 |
| Ensembl Protein ID | ENSP00000269138 |
| InterPro ID | IPI00018069 |
| Microarray Probe ID | 202816_s_at, Hs.153221.0.A1_3p_a_at, A_14_P111944 |
| EMBL ID | S79894, CR542103 |
| GO ID | 0005634 |
| RefSeq DNA Accession | NM_005637 |
| RefSeq Peptide Accession | NP_005628 |
| Entrez Gene ID | 6760 |
| Uniprot/Swissprot ID | Q15532, SSXT_HUMAN |
| OMIM ID | 600192 |
Here is an example search for the SS18 Gene:
Step 2 - Select a Gene
Once the gene you are searching for has displayed, click on the gene. If the gene you are in search for does not display, try another search string. If you continue to have trouble, search the Ensembl site at http://ensembl.org. If you can find it on the ensembl site, just copy the Ensembl Gene ID and search Bio::POD with that id. If you cannot find it on the Ensembl site, you will not be able to find it in Bio::POD while it is using Ensembl as a data source.
Selecting the gene will provide further details about the gene (chromosome location and number of alternative transcripts) as shown here:
Step 3 - Select a Transcript
Selecting the gene from the previous step likely returned one result displaying the chromosome location and number of transcripts. Click on the gene and you will then see the transcripts from which you may choose as shown here:
Click on the transcript you wish to use and then click "Open".
The gene you have chosen is now loading in Bio::POD. A status of completion displays at the bottom. This may take a while depending on the traffic between you and your Ensembl host and the size of the gene and its annotation.
Next, you may wish to go through the Navigating a Gene documentation.