Quick Start

Welcome to Bio::POD (Prioritized Oligo Design). With this program, you can:

• Load genes and related annotation from multiple sources into one location
• Efficiently select regions for screening using the Prioritization of Annotation Regions (PAR) algorithm.
• Design primer pairs with a graphical interface to Primer3

If this is your first time using Bio::POD, this quick start guide should help you get up and running as soon as possible. If you have not already installed Bio::POD, please refer to the installation instructions or contact Bio::Neos for assistance: http://bioneos.com/contact.php

The general progression of use for Bio::POD is as follows:

1. Load a Gene (also referred to as a Candidate)
2. Navigate through the gene (viewing exons, introns, annotation, etc.)
3. Inspect the results of the PAR algorithm and choose a region for screening
4. Design primer pairs
5. Save your work

If this is your first time using Bio::POD you may wish to start understanding the progression of use above by clicking each of the links and following the instructions. However, these steps are not always required and a user may wish to simply view the genomic structure and not design primer pairs or use some of the other features of Bio::POD that don't fit in a specified order:

• Add custom annotation
• Select and copy nucleotide and protein sequence
• Generate reports
• Use the Intro Wizard (for adding datasources and changing your preferences)

Click on the links above or choose a topic on the left to help you through the use of Bio::POD. As always, the Bio::Neos team is ready and interested in helping you if you have any troubles: http://bioneos.com/contact.php